Effects of acanthopanax senticosus injection on the learning and memory ability and the expression of f-actin in hippocampus of brain hypoxia rats
Zhang Yue,Qin Haozhi, Wang Ting， Niu Yuan,Ge Yao, Qiao Lixue, Du Ailin
Department of Physiology and Neurobiology and Sino-UK Joint Laboratory for Brain Function and Injury, Henan Provincial Key Laboratory of Brain research, Xinxiang Medical University，Xinxiang 453003, China
Abstract：ObjectiveTo explore the effects of acanthopanax senticosus injection(ASI) on the learning and memory abilities, and the expression of f-actin in hippocampus of cerebral anoxia rats. MethodsThirty-six SD male rats were randomly divided into three groups on average and then 24 rats were left for the other rats were not adapted to the environment. The model group rats (n=8) and the ASI group rats (n=8) were injected embolic fluid via carotis internal arteries to make cerebral model. The normal control rats (n=8) were injected equal dose saline. After 3 days, 90mg/（kg·d） ASI was intraperitoneal injected to ASI group rats for 7 days. The model group and the control group received equal dose saline via intraperitoneal injection every day for 7 days. After that，the learning and memory abilities were tested by Morris water maze detection. Subsequently, the expression of f-actin in hippocampus was measured. The latency, the traveling distance and the expression of f-actin were analyzed by one-way ANOVA and pairwise comparison used LSD method. ResultsFrom 1 to 4 days,the latency in the control group were （48.31±17.13）s, （30.25±16.02）s,(24.25±14.87)s and (14.09±9.06）s, in the model group were (54.94±13.09)s, （48.85±17.21）s,(38.41±21.01)s and (26.13±15.82）s, in the ASI group were （52.28±14.95）s, （36.66±16.90）s,(28.38±15.83)s and (17.03±12.14）s， the differences were statistically significant （F＝10.29, 48.19,156.64,168.74,all P＜0.05）.The traveling distance in the control group were （925.54±98.69）cm, （594.47±117.33）cm, (382.38±71.17)cm and (226.58.23.82）cm， in the model group were （1033.61±125.20）cm， （902.10±145.52）cm, (698.86±121.05)cm and (467.27±107.18）cm, in the ASI group were （930.36±124.51）cm， （662.17±145.40）cm, (412.42±73.98)cm and (233.36±32.48）cm, the differences were statistically significant （F＝27.30， 10.21, 5.58, 7.88， all P＜0.05）.The positive expressions of f-actin in hippocampus were 0.14±0.01, 0.09±0.01, 0.11±0.01 in control group, model group and ASI group, respctively, the difference was statistically significant （F＝104.37，P＜0.05）. ConclusionAcanthopanax senticosus injection can alleviate the symptoms and improve the abilities of learning and memory in brain hypoxia rats.
张越 秦浩志 王婷 牛源 葛瑶 乔立雪 杜爱林. 刺五加注射液对脑缺氧大鼠学习记忆能力和海马组织中纤维状肌动蛋白的影响[J]. 中华诊断学电子杂志, 2018, 6(4): 272-276.
Zhang Yue,Qin Haozhi, Wang Ting， Niu Yuan,Ge Yao, Qiao Lixue, Du Ailin. Effects of acanthopanax senticosus injection on the learning and memory ability and the expression of f-actin in hippocampus of brain hypoxia rats. zhzdx, 2018, 6(4): 272-276.
