Abstract：Objective To explore clinical significance of the provocative GH testing in clinical diagnosis of short children.MethodsRetrospective analysis of two hundred and fortythree cases of short children from February 2013 to April 2014，who were tested with levodopa and insulin test.Determing growth basic values and growth values after 30,60,90,120min of giving medicine.ResultsSixtyeight cases(27.98%) with complete lack of growth hormone,nintyeight patients (40.33%) with partial growth hormone deficiency,sixtysix cases (27.16%) with idiopathic short stature,seven cases (2.88%) with Turner syndrome,four cases with other causes (1.65%).Growth hormone deficiencies was the first cause.Comparing with the partial growth hormone deficiency,there was a significant difference(χ2=6.533，P<0.05).The peak(6.39±3.81)μg/L of growth hormone in Ldopa provocation tests was higher than the peak of growth hormone (5.02±3.57)μg/L in insulin provocation tests, but there was no statistically significant difference (t=1.640,P>0.05)；The peak of growth hormone in Ldopa provocation tests appeared in 60min,(one hundred and nineteen cases，48.97%),the peak of growth hormone in insulin stimulates test in 30min，60min respectively (one hundred and thirteen cases,46.50%；one hundred and twentyone cases，49.80%).ConclusionProvocation GH testing of levodopa and insulin can be used as diagnostic test of short children with growth hormone deficiency.
［1］Keller A，Donaubauer J，Kratzsch J，et al．Administration of arginine plus growth hormone releasing hormone to evaluate growth hormone (GH) secretory status in children with GH deficiency［J］. J Pediatr Endocrinol Metab,2007, 20(12)：1307-1314．
［2］中华医学会儿科学分会内分泌遗传代谢学组.矮身材儿童诊治指南［J］．中华儿科杂志. 2008,46(6)428-430．
［3］超楚生，唐二礼．激素不敏感综合征［M］．长沙：湖南科学技术出版社,1998:9．
［4］方圻．现代内科学［M］．北京：人民军医出版社,1998：25-27．
［5］Berg C,Meinel T,Lahner H,et al.Diagnostic utility of the glucagon stimulation test in comparison to the insulin tolerance test in patients following pituitary surgery［J］.Eur J Endocrinol,2010,162(3)：477-482．
［6］李永昶.小儿内分泌学［M］.北京：人民卫生出版社,1991:61.
［7］蒋健飞． 生长激素激发试验在矮小症诊治中的临床价值［J］.海南医学，2012，23(9)：40-41．
［8］颜纯，王慕．小儿内分泌学［M］．北京：人民卫生出版社，2006：95．
［9］陈蓉,王剑,李怀远,等.精氨酸与可乐定激发试验对儿童生长激素缺乏症的诊断价值［J］.现代检验医学杂志,2010,7(4):38-39.
［10］丁桂芝. 实用内分泌代谢病学［M］.武汉：科学技术出版社,1994:155.
［11］谢克俭，管瑜，留佩宁，等．不同生长激素激发试验诊断垂体性侏儒症的临床价值比较［J］．放射免疫学杂志，2003，16（2）：83-84．
［12］李燕虹，杜敏联，马华梅，等.吡啶斯的明与左旋多巴联合激发试验对儿童生长激素缺乏症的诊断价值［J］.中华内分泌代谢杂志，2004，20(3)：227-230.
［13］金薇，潘萍萍，袁新华.精氨酸、左旋多巴单日复合与两日序贯激发GH分泌的效果评价［J］.中国儿童保健杂志， 2011,19（7），651-652.
