质子磁共振波谱对急性肝损伤兔
肝脏脂质与葡萄糖代谢评估价值的研究

doi:10.3877/cma.j.issn.2095-655X.2024.03.004

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摘要目的探究质子磁共振波谱（1H-MRS）技术对兔急性肝损伤模型中肝脏脂质和葡萄糖代谢改变的评估价值。方法选择健康成年雄性新西兰大白兔20只，采用随机数字表法分为对照组(10只)和实验组(10只)。对照组腹腔注射生理盐水（1ml/kg）；实验组腹腔注射亚硒酸钠（1ml/kg），构建急性肝损伤模型。48h后，使用1.5T磁共振系统，采用单体素点分辨法波谱成像序列扫描兔活体肝脏。使用LCModel软件计算脂质、糖原和葡萄糖复合物（Glyu）和胆碱复合物（CCC）与水的相对峰值，并检测兔血清天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平，两组比较采用t检验。对兔肝脏标本进行苏木精-伊红（HE）染色，观察肝脏组织病理学变化。结果19只动物最终完成该实验过程，其中实验组9只，对照组10只。与对照组比较，实验组兔的lipid 13/lipid 09水平（5.21±1.83，7.17±2.56）、lipid 13/(lipid 21+lip 23)水平（6.52±0.82，7.35±2.33）均显著升高，而Glyu水平［（0.22±0.15）×10-2，（0.15±0.06）×10-2］和CCC水平［（0.09±0.03）×10-2，（0.07±0.03）×10-2］则显著下降，均差异有统计学意义（t=-2.31，-2.88，2.42，2.12；均P＜0.05）。实验组兔血清AST［（433.79±140.19）U/L］和ALT［（779.67±149.54）U/L］水平较对照组［（46.51±17.71）U/L，（69.44±20.79）U/L］均显著升高(t=5.46，13.92；均P＜0.05)。实验组兔肝脏出现肝小叶结构破坏、肝细胞坏死等急性弥漫性肝损伤的病理变化。结论利用亚硒酸钠可成功诱导兔急性肝损伤。1H-MRS技术可有效评估急性肝损伤的脂质与葡萄糖代谢改变，为临床无创诊断提供了新的思路。

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关键词 ：化学与药物性肝损伤, 磁共振波谱, 脂质, 葡萄糖

Abstract：ObjectiveTo investigate the value of proton magnetic resonance spectroscopy (1H-MRS) in assessing liver lipid and glucose metabolism alterations in a rabbit model of acute liver injury. MethodsTwenty healthy adult male New Zealand white rabbits were randomly divided into a control group (n=10) and an experimental group (n=10). The control group received intraperitoneal injection of normal saline (1ml/kg), while the experimental group received intraperitoneal injection of sodium selenite (1ml/kg) to establish an acute liver injury model. After 48 hours, the 1.5T magnetic resonance system was used to scan the live rabbit liver using a single-voxel point resolved spectroscopy sequence. LCModel software was employed to calculate the relative peaks of lipid, glycogen and glucose complex (Glyu), and choline-containing compounds (CCC) with water, and the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in rabbit serum were measured. Comparisons between the 2 groups were performed using t-tests. The rabbit liver specimens were stained with hematoxylin and eosin (HE) to observe pathological changes in liver tissues. ResultsNineteen animals ultimately completed the experimental process, with 9 in the experimental group and 10 in the control group. Compared to the control group, the experimental group showed significantly increased levels of lipid 13/lipid 09 (5.21±1.83, 7.17±2.56) and lipid 13/(lipid 21+lipid 23) (6.52±0.82, 7.35±2.33), while the levels of Glyu ［(0.22±0.15)×10-2, （0.15±0.06）×10-2］ and CCC ［(0.09±0.03）×10-2, （0.07±0.03)×10-2］ significantly decreased, all with statistically significant differences (t=-2.31, -2.88, 2.42, 2.12, all P＜0.05). Serum AST ［（433.79±140.19）U/L, （46.51±17.71）U/L］ and ALT ［（779.67±149.54）U/L, 69.44±20.79）U/L］ levels in the experimental group and control group were also significantly elevated (t=5.46, 13.92， all P＜0.05). The livers in the experimental group displayed pathological changes acute diffuse liver injury, such as destruction of the liver lobule structure and necrosis of liver cells. ConclusionsSodium selenite can effectively induced acute liver injury in the rabbit model. 1H-MRS technology is proved to be a valuable tool for evaluating the metabolic of lipid and glucose associated with acute liver injury, offering a novel perspective for clinical non-invasive diagnostic strategies.

Key words： Chemical and drug induced liver injury Magnetic resonance spectroscopy Lipid Glucose

收稿日期: 2024-04-12

基金资助:国家自然科学基金（31870981）；国家自然科学基金重点国际(地区)合作研究项目（82020108016)；2020 LKSF跨学科研究项目（2020LKSFBME06）；2020李嘉诚基金会跨学科研究项目（2020LKSFG05D）；广东省高水平大学发展计划临床医学重点学科项目（002-18120302）；广东省科技专项基金项目（STKJ2021148）；广东省基础与应用基础研究基金省企联合基金（2023A1515220187）；广东省基础与应用基础研究基金自然科学基金项目（2024A1515012267）

通讯作者: 吴仁华， Email：rhwu@stu.edu.cn

引用本文:

关计添尤克增耿义群孙树宜赖凌峰沈智威张晓磊周腾黄淮栋杨琳程焱吴烁华赵芝泓庄彩玉吴仁华 . 质子磁共振波谱对急性肝损伤兔肝脏脂质与葡萄糖代谢评估价值的研究[J]. 中华诊断学电子杂志, 2024, 12(3): 160-165.
Guan Jitian1,2, You Kezeng1,3, Geng Yiqun4, Sun Shuyi1, Lai Lingfeng1, Shen Zhiwei1,2, Zhang Xiaolei1,2, Zhou Teng5, Huang Huaidong1, Yang Lin1, Cheng Yan1, Wu Shuohua1, Zhao Zhihong1, Zhuang Caiyu1, . The value of proton magnetic resonance spectroscopy in assessing liver lipid and glucose metabolism alternations in rabbits with acute liver injury. zhzdx, 2024, 12(3): 160-165.

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